细胞培养操作流程

  记录一下细胞培养相关流程。

High throughput and multiplex localization of proteins and cells for in situ micropatterning using pneumatic microfluidics

  一种简单的、使用带有气动微结构的集成微流控装置,以高通量、几何动态和多图案方式高度可控地固定蛋白质和细胞。

Study 3D Endothelial Cell Network Formation under Various Oxygen Microenvironment and Hydrogel Composition Combinations Using Upside‐Down Microfluidic Devices

  一种倒置的微流控装置,用来研究不同的氧环境和水凝胶组成对3D内皮细胞网络形成的影响。

An integrated microfluidic 3D tumor system for parallel and high-throughput chemotherapy evaluation

  一种集成的气动操作和化学梯度生成器的微流控3D肿瘤系统,以用于平行、可控、动态和高通量的抗癌治疗研究。

A microfluidic platform for multi-size 3D tumor culture, monitoring and drug resistance testing

  一种具有完善的微孔阵列和简单的双层结构的微流控系统,可以大规模制造多尺寸的三维仿生肿瘤,并同时进行操作和正交分析。

Large-Scale Antitumor Screening Based on Heterotypic 3D Tumors Using an Integrated Microfluidic Platform

  一种基于气动的微流控芯片,用于产生异型肿瘤以用于大规模化疗药物筛选。

Heterotypic 3D tumor culture in a reusable platform using pneumatic microfluidics

  一种基于气动的微流控平台,可以在设备中生产各种异型和同型3D肿瘤阵列。

Monitoring Tumor Response to Anticancer Drugs Using Stable Three-Dimensional Culture in a Recyclable Microfluidic Platform

  一种基于气动微结构的微流控芯片,以用于对肿瘤反应的时间的、高通量的监测。

Controllable organization and high throughput production of recoverable 3D tumors using pneumatic microfluidics

  一种基于气动的微流控芯片,用于三维肿瘤形成与收集。

Dynamic trapping and high-throughput patterning of cells using pneumatic microstructures in an integrated microfluidic device

  一种整合气动微结构的微流控芯片用于动态捕获细胞及高通量细胞图案化。

3D cell culture 3D cell culturing 3D cell microarrays 3D culture 3D culture model 3D printing 3D spheroid 3D tumor culture 3D tumors 3D vascular mapping ACT ADV AUTODESK Abdominal wall defects Acoustofluidics Adipocyte Adipogenesis Adoptive cell therapy AirPods Alginate Anticancer Anticancer agents Anticancer drugs Apple Apriori Association Analysis August AutoCAD Autodock Vina Bio-inspired systems Biochannels Bioengineering Bioinspired Biological physics Biomarkers Biomaterial Biomaterials Biomimetic materials Biomimetics Bioprinting Blood purification Blood-brain barrier Bone regeneration Breast cancer Breast cancer cells Breast neoplasms CM1860 CRISPR/Cas9 system CSS CTC isolation CTCs Cancer Cancer angiogenesis Cancer cell invasion Cancer immunity Cancer immunotherapy Cancer metabolism Cancer metastasis Cancer models Cancer screening Cancer stem cells Cell adhesion Cell arrays Cell assembly Cell clusters Cell culture Cell culture techniques Cell mechanical stimulation Cell morphology Cell trapping Cell-bead pairing Cell-cell interaction Cell-laden gelatin methacrylate Cellular uptake Cell−cell interaction Cervical cancer Cheminformatics Chemotherapy Chimeric antigen receptor-T cells Chip interface Circulating tumor cells Clinical diagnostics Cmder Co-culture Coculture Colon Colorectal cancer Combinatorial drug screening Combinatorial drug testing Compartmentalized devices Confined migration Continuous flow Convolutional neural network Cooking Crawler Cryostat Curved geometry Cytokine detection Cytometry Cytotoxicity Cytotoxicity assay DESeq DNA tensioners Data Mining Deep learning Deformability Delaunay triangulation Detective story Diabetic wound healing Diagnostics Dielectrophoresis Differentiation Digital microfluidics Direct reprogramming Discrimination of heterogenic CTCs Django Double emulsion microfluidics Droplet Droplet microfluidics Droplets generation Droplet‐based microfluidics Drug combination Drug efficacy evaluation Drug evaluation Drug metabolism Drug resistance Drug resistance screening Drug screening Drug testing Dual isolation and profiling Dynamic culture Earphone Efficiency Efficiency of encapsulation Elastomers Embedded 3D bioprinting Encapsulation Endothelial cell Endothelial cells English Environmental hazard assessment Epithelial–mesenchymal transition Euclidean distance Exosome biogenesis Exosomes Experiment Extracellular vesicles FC40 FP-growth Fabrication Fast prototyping Fibroblasts Fibrous strands Fiddler Flask Flow rates Fluorescence‐activated cell sorting Functional drug testing GEO Galgame Game Gene Expression Profiling Gene delivery Gene expression profiling Gene targetic Genetic association Gene‐editing Gigabyte Glypican-1 GoldenDict Google Translate Gradient generator Gromacs Growth factor G‐CSF HBEXO-Chip HTML Hanging drop Head and neck cancer Hectorite nanoclay Hepatic models Hepatocytes Heterotypic tumor HiPSCs High throughput analyses High-throughput High-throughput drug screening High-throughput screening assays High‐throughput methods Histopathology Human neural stem cells Human skin equivalent Hydrogel Hydrogel hypoxia Hydrogels ImageJ Immune checkpoint blockade Immune-cell infiltration Immunoassay Immunological surveillance Immunotherapy In vitro tests In vivo mimicking Induced hepatocytes Innervation Insulin resistance Insulin signaling Interferon‐gamma Intestinal stem cells Intracellular delivery Intratumoral heterogeneity JRPG Jaccard coefficient JavaScript July June KNN Kidney-on-a-chip Lab-on-a-chip Laptop Large scale Lattice resoning Leica Leukapheresis Link Lipid metabolism Liquid biopsy Literature Liver Liver microenvironment Liver spheroid Luminal mechanics Lung cells MOE Machine Learning Machine learning Macro Macromolecule delivery Macroporous microgel scaffolds Magnetic field Magnetic sorting Malignant potential Mammary tumor organoids Manhattan distance Manual Materials science May Mechanical forces Melanoma Mesenchymal stem cells Mesoporous silica particles (MSNs) Metastasis Microassembly Microcapsule Microcontact printing Microdroplets Microenvironment Microfluidic array Microfluidic chips Microfluidic device Microfluidic droplet Microfluidic organ-on-a chip Microfluidic organ-on-a-chip Microfluidic patterning Microfluidic screening Microfluidic tumor models Microfluidic-blow-spinning Microfluidics Microneedles Micropatterning Microtexture Microvascular Microvascular networks Microvasculatures Microwells Mini-guts Mirco-droplets Molecular docking Molecular dynamics Molecular imprinting Monolith Monthly Multi-Size 3D tumors Multi-organoid-on-chip Multicellular spheroids Multicellular systems Multicellular tumor aggregates Multi‐step cascade reactions Myeloid-derived suppressor cells NK cell NanoZoomer Nanomaterials Nanoparticle delivery Nanoparticle drug delivery Nanoparticles Nanowell Natural killer cells Neural progenitor cell Neuroblastoma Neuronal cell Neurons Nintendo Nissl body Node.js On-Chip orthogonal Analysis OpenBabel Organ-on-a-chip Organ-on-a-chip devices Organically modified ceramics Organoids Organ‐on‐a‐chip Osteochondral interface Oxygen control Oxygen gradients Oxygen microenvironments PDA-modified lung scaffolds PDMS PTX‐loaded liposomes Pain relief Pancreatic cancer Pancreatic ductal adenocarcinoma Pancreatic islet Pathology Patient-derived organoid Patient-derived tumor model Patterning Pearl powder Pearson coefficient Penetralium Perfusable Personalized medicine Photocytotoxicity Photodynamic therapy (PDT) Physiological geometry Pluronic F127 Pneumatic valve Poetry Polymer giant unilamellar vesicles Polystyrene PowerShell Precision medicine Preclinical models Premetastatic niche Primary cell transfection Printing Protein patterning Protein secretion Pubmed PyMOL Pybel Pytesseract Python Quasi-static hydrodynamic capture R RDKit RNAi nanomedicine RPG Reactive oxygen species Reagents preparation Resistance Review Rod-shaped microgels STRING Selective isolation Self-assembly Self-healing hydrogel September Signal transduction Silk-collagen biomaterial composite Similarity Single cell Single cells Single molecule Single-cell Single-cell RNA sequencing Single‐cell analysis Single‐cell printing Size exclusion Skin regeneration Soft lithography Softstar Spheroids Spheroids-on-chips Staining StarBase Stem cells Sub-Poisson distribution Supramolecular chemistry Surface chemistry Surface modification Switch T cell function TCGA Tanimoto coefficient The Millennium Destiny The Wind Road Thin gel Tissue engineering Transcriptome Transfection Transient receptor potential channel modulators Tropism Tubulogenesis Tumor environmental Tumor exosomes Tumor growth and invasion Tumor immunotherapy Tumor metastasis Tumor microenvironment Tumor response Tumor sizes Tumor spheroid Tumor-on-a-chip Tumorsphere Tumors‐on‐a‐chip Type 2 diabetes mellitus Ultrasensitive detection Unboxing Underlying mechanism Vascularization Vascularized model Vasculature Visual novel Wettability Windows Terminal Word Writing Wuxia Xenoblade Chronicles Xin dynasty XuanYuan Sword Youdao cnpm fsevents miR-125b-5p miR-214-3p miRNA signature miRanda npm
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